Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs—commonalities and differences

6S-1 or 6S-2 RNA-templated pRNA synthesis at varying NTP concentrations. For details, see Materials and Methods. Lanes 1, 2, 9, 10: 5′-³²P-labeled RNA length markers mimicking a 6S-1 RNA-derived pRNA 8-mer (5′-GUUCGGUC, lanes 1,9) or a 14-mer (5′-GUUCGGUCAAAACU, lanes 2,10). Samples were analyzed by denaturing 25% PAGE. Asterisk at the right margin: These species appear only in the presence of 6S RNA; their nature is unknown and currently under investigation.