Tet-Trap, a genetic approach to the identification of bacterial RNA thermometers: application to Pseudomonas aeruginosa

Temperature-dependent expression of PA5194-sfGFP by P. aeruginosa cells. Images of PAO1 cells carrying plasmids pGM2013 (PA5194-sfGFP) or pGM2016 (RecA-sfGFP) were acquired on a Leica fluorescence microscope. The cells were sampled from cultures grown at the temperatures indicated between the panels as described in Materials and Methods. The same viewfield was captured in contrast phase and fluorescence imaging mode.