Structural insights into translational recoding by frameshift suppressor tRNA^SufJ

A-site binding of ASL^SufJ, ASL^Thr, tRNA^SufJ, and tRNA^Thr. Increasing concentrations of 70S ribosomes were programmed with A-site codons (A) ACC-A, (B) ACC-U, or (C) ACC-C and mixed with 2 nM [³²P] ASL^Thr (□/▵ dashed lines) or ASL^SufJ (▪/▴; solid lines). The presence of the antibiotic paromomycin is indicated by “+”. Similar binding experiments were also performed with tRNA^Thr (□/▵ dashed lines) or tRNA^SufJ (▪/▴; solid lines) with A-site codons (D) ACC-A, (E) ACC-U, or (F) ACC-C in the presence or absence of paromomycin (indicated by “+”).