FISH analysis reveals a single antisense lncRNA molecule in the nucleus for clones C5 and E3. (A) RNA-FISH to detect antisense lncRNAs that traverse the CMV-EGFP reporter cassette for the C5 clone. (Upper left) antisense lncRNA; (upper middle) DAPI staining; (upper right) merged. The bottom panel shows the enlarged image of the cells indicated by the color arrows. The probes were custom designed to tile the whole reporter cassette sequence. (B) RNA-FISH to detect antisense lncRNAs that traverse the CMV-EGFP reporter cassette for the E3 clone. (Upper left) antisense lncRNA; (upper middle) DAPI staining; (upper right) merged. The bottom panel shows the enlarged image of the cells indicated by the colored arrows. The probes were custom designed to tile the whole reporter cassette sequence.
