The role of antisense long noncoding RNA in small RNA-triggered gene activation

CMV-targeting shRNAs trigger TGA in the clones C5, D1, and E3. (A) Relative expression level of eGFP in CMV-EGFP clones C5, D1, and E3, as determined by RT-PCR and normalized to housekeeping gene HPRT1. In this study, all experiments were repeated three times independently and data are represented as mean ± SD. (B) The constitutive eGFP expression represented as the copy numbers of mRNA molecules per cell in three clones, D1, C5, and E3, as determined by RT-PCR.