miRNAome analysis of the mammalian neuronal nicotinic acetylcholine receptor gene family

Reciprocal relationship between miR-542-3p and nAChR β2 transcript levels in the mesocorticolimbic reward pathway following nicotine treatment. RNA was isolated from the indicated brain regions from animals treated with saline or nicotine as described in Materials and Methods. qRT-PCR was carried out for miR-494, miR-542-3p, nAChR α4, and nAChR β2 transcripts as indicated. Student's t-test was used for statistical analysis, (*) P < 0.05, (**) P < 0.01; n = 6.