FIGURE 4.
Effect of RRP45, PAN2, and CAF1/NOT complex depletion on mRNA decay. (A) Effects on the overall half-life distribution. Each unique ORF was placed in a half-life category as indicated in the key on the right. The numbers of ORFs in each category are displayed. (B) The half-life for each unique ORF after RNAi was divided by the half-life in wild type. The ORFs were then organized into categories depending on the wild-type half-life, and the average change in half-life after RNAi was calculated. The differences between the means of the categories for all RNAi lines were significant (one-way ANOVA on linearized data P-value <1 × 10−16).
