The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation

Depletion of RRP45 results in a 5′ read bias. Predominant polyadenylation and splice acceptor sites for as many mRNAs as possible were extracted from all our data sets. For each unique ORF, the read density was plotted across the entire predicted mRNA, then normalized to an mRNA length of 1000 nt. The average read density across all mRNAs was then plotted. Results for RNAi lines are compared with wild type, as indicated on the individual panels (A–E).