Spliced leader hybridization to Northern blots of the RNA samples sent for sequencing. RNA from different cells was prepared and subjected to Northern blotting with a spliced leader RNA 39mer oligonucleotide probe. Three similar samples were tested for each line; one typical lane is shown. A second set of lanes from wild-type (WT) and CNOT10 RNAi was shown in Färber et al. (2013). The arrow points to the spliced leader precursor RNA, and the asterisk marks signal from the 7SL (signal recognition particle) RNA left from a previous hybridization. The mRNA signal was quantitated in the region from ∼400 nt to 6 kb. The panel below shows the methylene blue signal from rRNA on the corresponding blot segments. The hybridization signals for individual RNAi lines differ because RNA from each cell line was run on a different gel. For further comparisons see Supplemental Figure S1.
