Sequences and binding properties of aptamers and RDE. Predicted secondary structures of Apt1 (A), Apt1-S (B), and RDE (C) and their SPR sensorgrams for AML1–Runt binding. (Red) The consensus DNA sequence for binding to the Runt domain in RDE. The indicated concentrations of the Runt domain were added to the aptamers or RDE immobilized on the sensor chip. The dissociation constant Kd was determined as the ratio of the association rate constant kon and the dissociation rate constant koff as follows: Kd = koff/kon. The mean Kd value ± SE (n = 3) is shown. The kon and koff values are shown in Tables 1 and 2. Kinetic parameters were determined using BIAevaluation software (BIAcore AB).
