FIGURE 2.
RNAs localized to either the vegetal or animal hemispheres are cross-linked to the same set of proteins. Internally radiolabeled PHAX (left), Vg1 (center), or An1 (right) RNA was added to whole-cell extract prepared from mixed-stage oocytes. Following UV irradiation, samples were treated with ribonuclease and then analyzed by SDS electrophoresis followed by autoradiography. A 20-fold excess of unlabeled, competitor RNA was added to individual samples, as indicated below each lane. Nonspecific RNA (ns) is a 356-nt transcript from the vector pBluescript KS.
