A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA

Comparison of the specificity of the conventional probe (Com) and the universal probe (Mod) using serially diluted single-stranded mature miRNAs and the corresponding miRNA precursors. Detection of miR-133b sequences using the conventional probe (A) or the universal probe (C). Detection of miR-146a sequences using the conventional probe (B) or the universal probe (D). (E) Ct values detected in A–D for 10⁹ template molecules. Error bars represent value range from mean of one experiment performed in duplicate. (NTC) No template control.