Comparison of the sensitivity of the conventional probe (Com) and the universal probe (Mod) using serially diluted single- and double-stranded RNA. (A) Detection of the GFP22 siRNA antisense strand derived from two double-stranded siRNA batches using both protocols; mean of duplicates with standard deviation. Detection of mature miR-133b as part of a double-stranded miRNA mimic (B) or as RNA single-strand (C). Detection of mature miR-146a as part of a double-stranded miRNA mimic (D) or as RNA single-strand (E). Error bars represent value range from mean of two independent experiments, each performed in duplicate. (NTC) No template control; (NEC) no reverse transcriptase control; (NPC) nonforward primer control.
