A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA

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FIGURE 1.
FIGURE 1.

Schematic diagram depicting the principle of stem–loop primer-based small RNA detection. The original design requires one TaqMan probe for each target RNA (lower left). The universal design uses a universal TaqMan-based probe which does not overlap with the target binding domain of the stem–loop primer and, hence, can be used to detect any target sequence (lower right).

This Article

  1. Published in Advance October 22, 2013, doi: 10.1261/rna.040501.113 RNA 19: 1864-1873

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