Kinetic mechanism of nick sealing by T4 RNA ligase 2 and effects of 3′-OH base mispairs and damaged base lesions

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FIGURE 2.
FIGURE 2.

Effects of 3′-OH N:purine mispairs on the kinetics of nick sealing. (Top panel) The distributions of radiolabeled AppNick intermediate and sealed product during the reaction of Rnl2 at a 3′ A:A mispaired nick are plotted versus time. Each datum is the average of three separate experiments. The curve fits are shown. (Bottom panel) The step 2 and step 3 rate constants for sealing nicks with the indicated 3′-OH N:purine configurations.

This Article

  1. Published in Advance October 24, 2013, doi: 10.1261/rna.041731.113 RNA 19: 1840-1847

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