Structural distortion of the decoding site by the P90W SmD substitution and the effect of streptomycin binding. (Gray) The wild-type 30S subunit (PDB accession code 4DR1) (Demirci et al. 2013); (blue) the P90W mutant; (purple) the P90L mutant; (green) the P90L/C1490U mutant. Wild type aligned with the P90W mutant (A) or P90L mutant (B) in crystal form 2. Wild type aligned with the P90W mutant (C) or the P90L mutant in (D) in crystal form 1. Alignment of wild type with the P90W mutant (E) or the P90L mutant (F), each cocrystallized with streptomycin. (G) Alignment of wild type and the P90W mutant after addition of streptomycin in preformed crystals in form 2. (H) Alignment of wild type with the P90L/C1490U SmI mutant in crystal form 1.
