The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

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FIGURE 5.
FIGURE 5.

Polyadenylation analysis. (A) Phenotypes of homozygous mutants grown at restrictive temperature. (n) Numbers of germlines tested. (B) Poly(A) tail analysis performed on L4 larvae. cDNAs of fem-3 3′ UTRs including poly(A) tails were resolved and analyzed by Southern blotting and sequencing. Poly(A) tails comprise between 12 and 50 adenosines.

This Article

  1. Published in Advance May 25, 2012, doi: 10.1261/rna.031237.111 RNA 18: 1385-1394

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