FIGURE 3.
Polyribosome analysis. (A) Cytoplasmic extracts from young adult fem-3(q95)/+ heterozygotes were resolved by sucrose density sedimentation. Absorbance trace at 260 nm with peaks corresponding to free RNAs and translationally silent messenger ribonucleoprotein, ribosomal subunits (40S, 60S), monosomes (80S), and polyribosomes. (B) Analysis of fem-3 transcripts in all fractions by RT-PCR and Southern blotting. (C) Wild-type (+) and gain-of-function-mutated (q95) fem-3 cDNAs were quantified by phosphoimager analysis. We found fewer wild-type transcripts throughout all fractions, except fraction #11 that coeluted with 40S.
