FEM-3 is specifically expressed in the sperm lineage. (A–C) Dissected adult germlines stained with DAPI, anti-RME-2, and anti-FEM-3 antibodies. RME-2 was found in the oocyte lineage. In contrast, FEM-3 is restricted to the sperm lineage. (D–F) Anti-FEM-3 staining challenged by FEM-3 peptides. The N-terminal peptide (FEM-3N) was used to produce the antibody. Panels D–F represent a detail of the spermatogenic region of a L4 larva. All micrographs were taken under the same light conditions and time of exposure (in msec). Only samples preincubated with FEM-3N showed strongly reduced FEM-3 staining in sperm. (G–I) Eight- to 12-blastomere embryos stained only for MOG-6 but not for FEM-3. Bar, 10 μm.
