FIGURE 4.
The cytoplasmic poly(A)-polymerase Gld-2 is required for normal adenylation of transcripts in the C. elegans germline. The adenylation state of four maternal mRNA egg-1, Pup-2, oma-2, and gpd-4 was analyzed in Bristol Normal (N2) and gld-2 mutant worms. In each case, the mRNA is short-tailed in the mutant, which reflects the inactive state. The adenylation state of the somatic transcript gpd-2 is not affected in a major way by the loss of Gld-2. Yeast total RNA (800 ng) from a deadenylase-deficient mutant (pan2Δ/ccr4-1) was spiked into the assay as ballast for the low concentration of worm RNA (200 ng). The yeast APQ12 panel is included to demonstrate equal ePAT efficiency across samples.
