Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS

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FIGURE 5.
FIGURE 5.

Binding of U2 SF3a/b proteins Prp11-EGFP and Cus1-EGFP to the spliceosome is made salt-sensitive by the catalytic activation. Affinity-purified BactΔPrp2 complexes were assembled on Atto647N-M3Act, carrying Prp11-EGFP (A) or Cus1-EGFP (B). The complexes were incubated with increasing salt concentration before (columns 1–3) or after (columns 4–6) complementation with Prp2, Spp2, and ATP.

This Article

  1. Published in Advance April 25, 2012, doi: 10.1261/rna.033316.112 RNA 18: 1244-1256

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