Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

Displacement of Bud13-EGFP from the spliceosome during Prp2-mediated catalytic activation. Affinity-purified BactΔPrp2 complexes assembled on Atto647N-M3Act, carrying Bud13-EGFP (columns 1–3), were complemented with Prp2, Spp2, and ATP (columns 4–6). After incubation, KCl was added in increasing concentrations, and dcFCCS was performed as above.

This Article

  1. Published in Advance April 25, 2012, doi: 10.1261/rna.033316.112 RNA 18: 1244-1256

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA