Properties of small rRNA methyltransferase RsmD: Mutational and kinetic study

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FIGURE 5.
FIGURE 5.

(A) ITC of RsmD interaction with small ligands. Shown are ITC profiles for SAM interactions with RsmD (c) and its mutants: PP129-129AA (d), D58A (e), and D58A/PP129-129AA (f), and of RsmD interactions with Sin (a) and SAH (b) at 25°C in the buffer 20 mM Hepes K pH 7.5, 5 mM Mg(OAc)2, 200 mM NH4OAc, 10% glycerol, 5 mM DTT. (B) Reverse transcription analysis of the total rRNA extracted from the knockout RsmD strain transformed with the plasmids coding for the following rsmD mutants: (1) PPF128-130AAS, (2) F130S, (3) PP128-129AA, (4) D58A, (5) D58A/PP128-129AA, (6) no mutation (control).

This Article

  1. Published in Advance April 25, 2012, doi: 10.1261/rna.032763.112 RNA 18: 1178-1185

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