FIGURE 3.
Interaction of 30S ribosomes devoid of G966 methylation with the methyltransferase RsmD in the presence of sinefungin monitored by chemical and enzymatic footprinting. Lanes A, C, G, U correspond to sequencing lanes in the presence of corresponding ddNTP; UN corresponds to untreated 30S subunits of 30S·RsmD complex; DMS, Keth, T1, and T2 each correspond to treatment with dimethyl sulfate, kethoxal, and RNases T1 and T2, respectively; +/− indicates the preincubation of unmethylated at G966 30S subunits with RsmD and sinefungin. Protected bases are marked by arrows.
