Complex formation of RsmD with 30S subunits devoid of G966 methylation. (A) Fractionation of 30S·RsmD complex by sucrose density gradient ultracentrifugation. Shown is the optical density at 260 nm vs. fraction number starting from 30% to 10% sucrose. (B) Immunodetection of RsmD in the same fractions of sucrose density gradient. Lane K contains 1 pmol pure RsmD. (C) Immunodetection of ribosomal protein S4 in the same fractions of sucrose density gradient. Lane K contains 1 pmol total 30S proteins. (D) Efficiency of RsmD complex formation with 30S subunits and 70S ribosomes devoid of G966 methylation. Efficiency of complex formation was monitored by immunoblotting of the corresponding sucrose density centrifugation fractions.
