Small RNA-sequencing from Dicer WT and KO cells transfected with hsDicer constructs. (A) Summary of total library reads mapped to mature miRNAs (blue), pre-miRNAs (orange), or non-miRNA loci (green). One mismatch was allowed for mapping. Mature miRNAs were identified as reads with an exact 5′ end and a ±1-nt length variation at the 3′ end compared with previously published annotations (Chiang et al. 2010). (B) Reads mapping to mature 5p or 3p arms of precursors. (C) Expression of the let-7 family of miRNAs. (B,C) miRNA composition was calculated as a percentage of total reads mapped, with 0 or 1 mismatch, to the mouse genome (mm9). The sequencing was performed in biological duplicate. The results shown are the average of the duplicate libraries.
