Multiple miRs contribute to LATS2 3′ UTR mediated translational repression in BJ cells and AGS cell line. (A) Average precision of the relative luciferase assay using miR-Sens reporter in BJ cells. The precision of the measure for the Renilla, the Firefly luciferase, and the ratio of RL/FL luciferase signal was calculated based on three independent transductions for each miR-Sens vectors from Figure 2A and data not shown (n = 6 constructs). The average precision and its standard deviation were subsequently determined. (B) Effect of the miR-31 site mutation in LATS2 3′ UTR on the relative luciferase activity in MDA-MB-231 cells. miR-31-5p sensor was used as positive control for miR-31 expression. miR-31 was overexpressed with miR-Vec blast in MDA-MB-231 cell line. (C) Same as in B in AGS cell line. miR-373-3p was used as a positive control for miR-373 expression. miR-371-2-3 and miR-31 are constitutively expressed in AGS cell line. (D) Enlarged results from C. In these experiments, only the relative luciferase activity of the miR-Sens empty vector in control cells is normalized to 100%.
