miR-Sens—a retroviral dual-luciferase reporter to detect microRNA activity in primary cells

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FIGURE 2.
FIGURE 2.

Efficient detection of miR expression with miR-Sens sensors. (A) Relative luciferase activities for miRNA sensors in BJ cells. (B) Absolute levels of Firefly and Renilla luciferase activities from A, indicating similar retroviral titer and transduction efficiency for various miR-Sens vectors. (C) Relative luciferase activities for miRNA sensors in HMECs. (D) Absolute levels of Firefly and Renilla luciferase activities from C, indicating that a change in the viral titer does not compromise the specificity of the miR-Sens vector. (E) Correlation between constitutive miRNAs expression and the relative luciferase activity of miR-Sens sensors in BJ cells (n = 10). Five nonexpressed miRNAs are used as negative controls (hsa-miR-29b-2-5p, hsa-miR-29c-5p, 302c-3p, 372-3p, and 373-3p).

This Article

  1. Published in Advance March 14, 2012, doi: 10.1261/rna.031831.111 RNA 18: 1091-1100

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