The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B

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FIGURE 4.
FIGURE 4.

SRp20 and Tra2-β overexpression reverses digitoxin effect on coregulated alternative exons. (A) Digitoxin-induced repression of ZNF207 exon 9 is reversed by SRp20 expression. (B) RIPK2 exon 2 repression is rescued by Tra2-β expression. (C,D) Digitoxin induced repression of APP exon 8 is rescued by expression of exogenous SRp20, while the same overexpression reduced digitoxin-induced enhancement of FN1 exon 33. (E,F) Flag-tagged overexpression of SRp20 and Tra2-β in HEK293 cells, respectively.

This Article

  1. Published in Advance March 28, 2012, doi: 10.1261/rna.032912.112 RNA 18: 1041-1049

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