Computational prediction of efficient splice sites for trans-splicing ribozymes

Transcription efficiencies of ribozymes analyzed in this study. All values are relative to the transcription efficiency of the ribozyme targeting splice site 97. Transcription efficiencies were determined from band intensities of internally [³²P]-labeled ribozymes, which had been transcribed in vitro and separated by denaturing polyacrylamide gel electrophoresis. The targeted splice site is indicated at the bottom of each bar. Error bars are standard deviations from seven independent transcriptions.