Kinetics of tRNA folding monitored by aminoacylation

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FIGURE 5.
FIGURE 5.

(A) Secondary structure of E. coli tRNA1Gln(UUG) indicating the positions of post-transcriptional modifications. The base modification at position U34 includes the 2-thio group as depicted, but its full identity is unknown. (B) Glutaminylation of native, fully modified E. coli tRNA1Gln. Diamonds indicate the time-course of aminoacylation after optimized folding at 21°C, circles indicate the time-course after flash-cooling at 0°C, and inverted triangles represent tRNA that was flash-cooled at 0°C and renatured at 21°C for 30 min prior to addition of enzyme. See text for details. (C) Glutaminylation of unmodified E. coli tRNA1Gln. Symbols are to be interpreted as in panel B.

This Article

  1. Published in Advance January 27, 2012, doi: 10.1261/rna.030080.111 RNA 18: 569-580

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