Detection, genomic context, and sequence of sRNAs isolated from the S. pneumoniae TIGR4 strain. (A) srn176, (B) srn308, (C) srn029, (D) srn142, and (E) srn299. The arrows indicate the size of the sRNA detected by Northern blotting, which agrees with the size predicted by sequencing. (C) RNA molecular weight marker. (Shadowed rectangles) The coordinates in the genome and the flanking genes for each sRNA. (Open circles) A potential intrinsic terminator. The nucleotide sequence of each sRNA (shadowed) is also shown, where the putative promoters at −10, or −10 extended, and −35 boxes are labeled with an empty rectangle, and the consensus nucleotides are depicted in bold letters. Potential terminators are shown in italic, bold letters, and putative ribosomal binding sites (RBS) and start codons of downstream genes (M) are in bold, underlined letters.
