Influence of the 5′ UTR of the reporter mRNA on frameshift efficiency with a dual-luciferase system in Jurkat T-cells. (A) The Fluc/Rluc ratio represents the relative frameshift efficiency corresponding to the different 5′ UTRs inserted upstream of the dual-luciferase coding sequences. The Fluc/Rluc ratios are presented relative to the p5′UTR-HIV construct, with the complete 5′ UTR of HIV-1 full-length mRNA, which is arbitrarily set at 100%. Each value represents the mean ± SEM of at least three independent triplicate experiments. (B) Effect of the 5′ UTR on translation efficiency. The Rluc activities were divided by the corresponding mRNA levels that were quantified by qPCR. The Rluc/mRNA ratios are presented relative to the p5′UTR-HIV construct, which is arbitrarily set at 1. Rluc/mRNA ratios were measured in triplicate, and one representative experiment is shown.
