A 5′-terminal phosphate is required for stable ternary complex formation and translation of leaderless mRNA in Escherichia coli

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

Analysis of cI-lacZ mRNA by Northern hybridization. Autoradiogram of size-fractionated in vivo RNA probed for cI-lacZ mRNA. Arrows indicate predicted positions of full-length mRNA (FL), 23S, and 16S rRNA. Lanes are labeled according to total in vivo RNA extracted from the E. coli RFS859 host strain (RFS) or the host strain containing plasmids expressing cI-lacZ fusions described in Table 1.

This Article

  1. Published in Advance January 30, 2012, doi: 10.1261/rna.027698.111 RNA 18: 508-518

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA