A 5′-terminal phosphate is required for stable ternary complex formation and translation of leaderless mRNA in Escherichia coli

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FIGURE 1.
FIGURE 1.

Hammerhead ribozyme sequence, proposed structure, and site of cleavage (arrow). Shaded regions represent areas of base pairing. (A) Structure resulting in leaderless cI-lacZ mRNA with a 5′-OH after cleavage. (B) Structure resulting in lac-leadered cI-lacZ mRNA with a 5′-OH after cleavage. Sequence in bold identifies the beginning of the cI coding sequence (A) or the lac-untranslated leader sequence (B).

This Article

  1. Published in Advance January 30, 2012, doi: 10.1261/rna.027698.111 RNA 18: 508-518

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