Deep-sequencing of endothelial cells exposed to hypoxia reveals the complexity of known and novel microRNAs

Novel-112 and novel-144 affect hypoxia-induced growth arrest. HUVECs were transfected with novel-112 or novel-144 mimics or with a control scramble sequence. The next day (T0), cells were exposed to 1% hypoxia for the indicated time and counted. Following hypoxia, novel-112 expression significantly decreased (A) and novel-144 expression significantly increased (B) cell number compared with that of the control (n = 16; *P < 0.003; ***P < 0.0005).