FIGURE 4.
Pipeline of genome mapping. Flowchart describing data analysis pipeline designed for the identification of novel potential miRNA candidates. Numbers in italics indicate the amount of remaining reads/hits/miRNAs after each filtering step, averaged across all four libraries. Filtering threshold for alignment length was set at ≥19 nt, allowing not more than one mismatch and one sequencing error. After final overlap with a hairpin prediction database, more than 270,000 hits remained, representing 511 candidates for putative novel miRNA locations.
