FIGURE 1.
Analysis pipeline of annotated miRNAs. Flowchart describing data analysis pipeline designed for the identification of mature and star sequences of annotated miRNAs, their isoforms, and novel miRNA star sequences. The number of remaining reads/hits/miRNAs after each filtering step, averaged across all four libraries, is indicated in italics. The high-stringency filter of alignment (step 5) consisted in minimum alignment length of 17 nt, with a maximum of one mismatch and three sequencing errors. Reads with sequencing errors in the first 15 nt or mismatches in the seed region were excluded.
