FIGURE 3.
Tudor-SN does not localize to P-bodies. (A) HeLa cells were transiently transfected with an expression vector for mC–TSN. After 24 h the cells were cultured in the absence (a–c) or presence of arsenite (d–f) before processing for visualization of mC–TSN (red; a,d) and G3BP (green; b,e) using confocal microscopy. DAPI staining is blue (Merge; c,f). (B) HeLa cells were transiently transfected with an expression vector for mC–TSN. After 24 h the cells were cultured in the absence (a–c) or presence of arsenite (d–f) before processing for visualization of mC–TSN (red; a,d) and Dcp-1 (green; b,e) using fluorescence microscopy. DAPI staining is blue (Merge; c,f). Bar, 10 μm.
