La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

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FIGURE 7.
FIGURE 7.

Slf1p stabilizes specific mRNA targets. (A) Scheme of RNA decay measurements. Transcription was halted by the addition of thiolutin to slf1Δ cells expressing either WT (pSLF1) or mutant (pLAM-m) Slf1 as a control. (B) Difference of remaining mRNA levels (pSLF1/pLAM-m expressing cells) of Slf1p targets (black) and nontargets (white) at various time points in minutes after thiolutin addition. Representative experiments are shown with standard deviations from three replicate measurements.

This Article

  1. RNA 18: 449-461