La-motif–dependent mRNA association with Slf1 promotes copper detoxification in yeast

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FIGURE 2.
FIGURE 2.

Copper resistance of SLF1 overexpressing cells depends on a functional LAM. (A) Yeast cells harboring a plasmid for gal-dependent expression of SLF1, SRO9, LHP1, or the empty vector were serially diluted (1:10) and spotted on SCgal-Ura plates containing indicated concentrations of CuSO4. (B) Same assay comparing WT (pSLF1) and Slf1 La-mutant (pLAM-m) expressing cells. Pictures were taken after incubation of plates for 3–5 d at 30°C.

This Article

  1. Published in Advance January 23, 2012, doi: 10.1261/rna.028506.111 RNA 18: 449-461

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