The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA

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FIGURE 5.
FIGURE 5.

Phylogeny of the COG1901 family and structural comparison of Mj1640 dimer with VV2_1434 dimer. (A) Unrooted Bayesian tree of 42 bacterial and 72 archaeal proteins identified as COG1901 in GenBank and are listed in Supplemental file 1. For clarity, only taxa from major clades are labeled. The scale bar indicates the average number of substitutions per site. Numbers at branches represent posterior probabilities as inferred by Mr. Bayes; for clarity, only major branches are labeled. The division between Bacteria and Archaea is well supported. (*) A. borkumensis SK2; (+) S. baltica OS155, OS183, and OS185. (B) Ribbon representation of Mj1640 dimer (left); Electrostatic surface potential presentation of Mj1640 dimer (right), with blue and red colors corresponding to positively and negatively charged patches, respectively. (C) Ribbon (left) and electrostatic surface potential representation (right) of VV2_1434 dimer.

This Article

  1. RNA 18: 421-433