Predicted pseudouridine modifications in ribosomal RNA based on complementary guide regions in Pyrobaculum H/ACA-like sRNAs (Pyrobaculum aerophilum domains shown). Structurally equivalent pseudouridine positions from model species shown (boxed) (Fournier website: people.biochem.umass.edu/fournierlab/3dmodmap/) (Muller et al. 2008). Position of complementary sRNA guide regions depicted by gray lines. Pseudouridine predictions supported only by conservation in other species shown as plain Ψ, and those supported by experimental evidence in Pyrobaclum as circled Ψ. The single predicted pseudouridine site with negative experimental data (16S-U762) depicted as circled U. Secondary structure map adapted from Thermoproteus tenax and Thermococcus celar, with permission (Gutell website: http://www.rna.icmb.utexas.edu/) (Cannone et al. 2002).
