Probing the substrate specificity of the bacterial Pnkp/Hen1 RNA repair system using synthetic RNAs

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Repair of cleaved full-length and tRNA deletion mutants depicted in Figure 1 by AvaPnkp/Hen1. (A,C) Time course of RNA repair of tRNAAsp and its deletion derivatives. (B,D) Time course of RNA repair of tRNAArg and its deletion derivatives. The positions of RNA substrates and repair products are marked with numbers representing their sizes in nucleotides. T indicates time in minutes.

This Article

  1. RNA 18: 335-344