
Temporal expression of RHA and type I collagen in activation of hepatic stellate cells (HSCs). (A) Temporal expression of RHA protein. HSCs were isolated from rat livers and plated into uncoated plastic dishes. The cells were cultured for the indicated time periods, collected, and analyzed for expression of RHA, collagen α1(I), α-smooth muscle actin, and tubulin by Western blot. (B) Temporal expression of RHA mRNA. Same experiment as in A, except total RNA was analyzed by radiolabeled RT-PCR for RHA and actin (ACT) mRNAs.










