Association of collagen mRNAs with RHA in human lung fibroblasts (HLFs). (A) HLFs (lanes 1,3) and HLFs transduced with adenovirus expressing LARP6 (lane 2) were used for IP with anti-RHA antibody (lanes 1,2) or anti-fibronectin antibody (lane 3). The IP material was analyzed for the presence of collagen α1(I), collagen α2(I), and actin (ACT) mRNAs by radiolabeled RT-PCR. (Bottom panel) Expression of the mRNAs in the input material. (B) Analysis of collagen mRNAs in the IP by real-time PCR. The amount of immunoprecipitated mRNAs is presented as the percentage of the amount in the input. The bars representing the standard error of the mean are indicated. (C) Protein expression of endogenous RHA and transduced LARP6 from the input material. Loading control: actin (ACT).
