FIGURE 1.
Cryo-EM images of RNAs in films of freely suspended vitrified solution. (A,C) Fourier band-pass filtered cryo-EM images of 1523-nt (A) and 975-nt (C) RNAs in vitrified 10 mM TE buffer. Individual RNA molecules with different configurations, suspended in random orientations, are seen as dark branched objects. (B,D) Traced skeletons (see Materials and Methods) of the molecules in panels A and C. For each RNA, the inset (E,F) depicts a hundred traced projections superimposed with their centers of mass in registry. Scale bars, 60 nm. (For more traces, see Supplemental Fig. S2.)
