The C-terminal domains of RBFOX1 and RBFOX 2 interact specifically with hnRNP H1, RALY, and TFG. (A) HeLa cells were transfected with MS2 or MS2-Fox1C. The proteins were immunoprecipitated from whole-cell lysates using Protein G Dynabeads coated with T7 monoclonal antibody. The immunoprecipitated proteins were separated on a gradient SDS–polyacrylamide gel and stained with Coomassie Blue. (B,C) IP using T7 antibody cross-linked to Protein G Dynabeads and Western blotting with Flag, hnRNP H1, RALY, and TFG antibodies. IPs were performed either with (+) or without (−) nuclease treatment. Whole-cell lysates, post-IP lysates, and IPs were blotted with the indicated antibodies. (B) HeLa cells transfected with MS2 or MS2-Fox1C. (C) HeLa cells transfected with MS2 or MS2-Fox2C. (*) Nonspecific bands.
