Regulation of release factor expression using a translational negative feedback loop: A systems analysis

Premature stop codon contexts well-recognized by eRF1 allow efficient feedback control of eRF1 and resist perturbation through enhanced transcription. Model 3, describing the regulation of eRF1 expression in the sup45 SUQ5 mutants, was used to simulate the effect of increased transcription of the nonsense sup45 allele on eRF1 expression levels, with varying affinity of the release factor for the premature stop codon. (●) K_M = 0.002 μM; (■) K_M = 0.008 μM; (▴) K_M = 0.014 μM; (▾) K_M = 0.024 μM.