Novel insights into the architecture and protein interaction network of yeast eIF3

Map of interactions between eIF3 subunits. The stable subcomplexes that could be separated by SEC are depicted by dashed lines. The interaction of Nip1 with Prt1-Tif34-Tif35 subcomplex is represented by a solid line around Prt1-Tif34-Tif35. Binding of Nip1^370-570 to Prt1^CTD was observed by Valasek et al. (2002), while the binding of Tif35^CTD to Prt1^CTD was concluded from indirect observations. Therefore these two interactions are represented by a different color (gray dashed lines).